使用基于 iTRAQ 技术的蛋白质组学分
德孚肿瘤靶标和治疗期刊发表了最新文章使用基于iTRAQ技术的蛋白质组学分析将HSPA8确定为子宫内膜癌的候选生物标志物。其内容摘要如下：
Abstract:Althoughthereareadvancesindiagnostic,predictive,andtherapeuticstrategies,discoveringproteinbiomarkerforearlydetectionisrequiredforimprovingthesurvivalrateofthepatientswithendometrialcarcinoma.Inthisstudy,weidentifyproteinsthataredifferentiallyexpressedbetweentheStageIendometrialcarcinomaandthenormalpericarcinoustissuesbyusingisobarictagsforrelativeandabsolutequantitation(iTRAQ)-basedproteomicanalysis.Totally,wescreened1,proteins.Amongthem,proteinsweresignificantlyoverexpressed,and30weresignificantlydownexpressedinendometrialcarcinoma.Usingthebioinformaticsanalysis,weidentifiedalistofproteinsthatmightbecloselyassociatedwithendometrialcarcinoma,includingCCT7,HSPA8,PCBP2,LONP1,PFN1,andEEF2.WevalidatedthegeneoverexpressionofthesemoleculesintheendometrialcarcinomatissuesandfoundthatHSPA8wasmostsignificantlyupregulated.WefurthervalidatedtheoverexpressionofHSPA8byusingimmunoblotanalysis.Then,HSPA8siRNAwastransferredintotheendometrialcancercellsRL-95-2andHEC-1B.ThedepletionofHSPA8siRNAssignificantlyreducedcellproliferation,promotedcellapoptosis,andsuppressedcellgrowthinbothcelllines.Takentogether,HSPA8playsavitalroleinthedevelopmentofendometrialcarcinoma.HSPA8isacandidatebiomarkerforearlydiagnosisandtherapyofStageIendometrialcarcinoma.
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